Stem Cells Across The Curriculum

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Cellular Reprogramming (iPSC) Video

This video was created by Bruce Conklin and the Gladstone Institutes and reviews nuclear reprogramming protocols for inducing pluripotency and multipotency (Video1: iPSC to Direct Reprogramming). Permission to post for educational use and non-commercial use received from copyright holder, The Gladstone Institutes, on January 20, 2012 from Jeanette Borzo, Chief Communications Officer at the Gladstone Institutes: jeanette.borzo@gladstone.ucsf.edu  and videos were received from Elena Lewis Administrator for Bruce Conklin, M.D., Cardiovascular Disease, Gladstone Institutes elana.lewis@gladstone.ucsf.edu.

Resources

In 1942, Conrad Waddington coined the term “epigenetics” to describe the complex relationship between genes and their environments and applied this notion to development proposing irreversible paths of cell differentiation later referred to as the Waddington Landscape. This visual metaphor of rivers, valleys, and mountains was meant to depict stable cell states and barriers that restricted cell specific lineages. An historical overview and contemporary analysis of this model in the context of new findings in stem cell biology are addressed in the following articles and dynamic videos. A paradigm shift is underway with respect to cellular reprogramming where the path of cell differentiation is no longer considered a series of linear steps for which there is no reversal. Removing cells from their niche or stimulating them to differentiate and divide with external factors has led to a new field of research and regenerative medicine as depicted in the New York Times ECM video and additional videos in the Video Guide titled Stem Cell Therapies: Hope & Hype.

  1. Animations: These videos were created by Bruce Conklin and the Gladstone Institutes and reviews nuclear reprogramming protocols for inducing pluripotency and multipotency (Video1: iPSC to Direct Reprogramming), and functionality of differentiated cells post induction (Video 2: CM-RBC and Video 3: M-chambered iPS-CV). Permission to post for educational use and non-commercial use received from copyright holder, The Gladstone Institutes, on January 20, 2012 from Jeanette Borzo, Chief Communications Officer at the Gladstone Institutes: jeanette.borzo@gladstone.ucsf.edu  and videos were received from Elena Lewis Administrator for Bruce Conklin, M.D., Cardiovascular Disease, Gladstone Institutes elana.lewis@gladstone.ucsf.edu
  2. Visual Review: Hochedlinger, K. & Plath, K.  2009. Epigentic reprogramming and induced pluripotency. Development. 136:509-523. Link to ppt slides.
  3. Video: Inwin,D (Director). 2008. Stem Cells Breakthrough. Link  NOVA Stem Cell Now (Producer).
  4. Animation: Worldstemcell. March 9, 2010. Cellular Reprogramming Animation. YouTube. 2:46 minute animation reviewing somatic cell gene expression and the Yamanaka reprogramming factors in the context of induced pluripotent stem cell protocols. Link
  5. Film: Hardie, A. & Blackburn, C. 2012. Stem Cells The Future: An Introduction to iPS cells. Link
  6. Video: Singh, V. Sept. 17, 2012. Extracellular Matrix. New  York Times. Video/Health (4:23 min).  Eurostemcell. Link. 15 min video reviewing the identification of the Yamanaka reprogramming factors.
  7. Leading Edge Review:  Goldberg, A. et al. 2007. Epigenetics: A landscape takes shape. Cell.  128(4):635-638. Link
  8. News Feature: Powell, K. 2005. Stem cell niches: It’s the ecology, stupid! Nature. 435 (7040): 268-270. Link
  9. Research Highlight: Baumann, K. 2010. Stem cells: Holding onto the memories. Nature. 11(9):593. Link